A list of regular Neighbours characters from the 2000 season, organised by the number of episodes they were featured in. In 2000, a total of 235 episodes were broadcast.

1. Susan Kennedy (134 episodes)
2. Harold Bishop (128 episodes)
3. Karl Kennedy (126 episodes)
4. Stephanie Scully (125 episodes)
5. Toadfish Rebecchi (124 episodes)
6. Lyn Scully (119 episodes)
=6. Felicity Scully (119 episodes)
8. Tad Reeves (116 episodes)
9. Joe Scully (115 episodes)
=9. Lou Carpenter (115 episodes)
11. Lance Wilkinson (114 episodes)
12. Paul McClain (112 episodes)
13. Libby Kennedy (108 episodes)
=13. Drew Kirk (108 episodes)
15. Joel Samuels (106 episodes)
16. Teresa Bell (101 episodes)
17. Michelle Scully (88 episodes)
18. Dione Bliss (87 episodes)
19. Madge Bishop (75 episodes)
20. Anne Wilkinson (30 episodes)
21. Daniel Fitzgerald (27 episodes)*
22. Louise Carpenter (25 episodes)
23. Amy Greenwood (22 episodes)
24. Bill Kennedy (18 episodes)
25. Darcy Tyler (8 episodes)

* Although only a guest character during the 2000 season, this character would later return as part of the regular cast